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bacterial expression vector pet21a  (Addgene inc)


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    Addgene inc bacterial expression vector pet21a
    Bacterial Expression Vector Pet21a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bacterial+expression+vector+pet21a/pIC246+(Plasmid+%2369740)/pm40054855__ja4c17815_si_001-14-6-11
    Average 94 stars, based on 3 article reviews
    bacterial expression vector pet21a - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Photochemistry of Receptor-Bound Flavin Resolved in Living Human Cells by Infrared Spectroscopy.
    Article Snippet: .. The amplicon was cloned into the bacterial expression vector pET21a (+) (Addgene #69740-3) using the restriction enzymes NdeI and XhoI. ..

    Clone Assay:

    Article Title: Photochemistry of Receptor-Bound Flavin Resolved in Living Human Cells by Infrared Spectroscopy.
    Article Snippet: .. The amplicon was cloned into the bacterial expression vector pET21a (+) (Addgene #69740-3) using the restriction enzymes NdeI and XhoI. ..

    Expressing:

    Article Title: Photochemistry of Receptor-Bound Flavin Resolved in Living Human Cells by Infrared Spectroscopy.
    Article Snippet: .. The amplicon was cloned into the bacterial expression vector pET21a (+) (Addgene #69740-3) using the restriction enzymes NdeI and XhoI. ..

    Plasmid Preparation:

    Article Title: Photochemistry of Receptor-Bound Flavin Resolved in Living Human Cells by Infrared Spectroscopy.
    Article Snippet: .. The amplicon was cloned into the bacterial expression vector pET21a (+) (Addgene #69740-3) using the restriction enzymes NdeI and XhoI. ..



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    Bacterial strains and plasmids used in this study

    Journal: Archives of biochemistry and biophysics

    Article Title: Biochemical characterization of bacterial FeoBs: A perspective on nucleotide specificity

    doi: 10.1016/j.abb.2020.108350

    Figure Lengend Snippet: Bacterial strains and plasmids used in this study

    Article Snippet: : F − omp T hsd S B (r B − , m B − ) gal dcm (DE3) [ 41 ] Plasmids pET21a Bacterial expression vector Novagen pEcNFeoB pET21a carrying the cytosolic domain of E. coli FeoB This study pSmNFeoB pET21a carrying the cytosolic domain of S. mutans FeoB This study pEcNFeoB-N11A pEcNFeoB with a N11A mutation This study pSmNFeoB-N11A pSmNFeoB with a N11A mutation This study Open in a separate window Bacterial strains and plasmids used in this study.

    Techniques: Plasmid Preparation, Clone Assay, Expressing, Mutagenesis